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. Author manuscript; available in PMC: 2018 Oct 15.
Published in final edited form as: J Immunol. 2018 Mar 9;200(8):2615–2626. doi: 10.4049/jimmunol.1700429

Fig.7. No differences in myeloid cell compartments between FcγRI/II/III/IV-/- and WT C57BL/6 control mice.

Fig.7

a. Spleens of three months old FcγRI/II/III/IV-/- and WT C57BL/6 mice were incubated with Liberase, and single cell suspensions were labeled and analyzed by flow-cytometry. Graphs show the percentage CD8 positive or negative cells of CD11c+/CD19-/ B220-/7-AAD- cells. Three mice per group. Data shown are from one out of two experiments with similar results.

b. Spleens of three months old FcγRI/II/III/IV-/- and WT C57BL/6 mice were incubated with Liberase, and single cell suspensions were labeled and analyzed by flow-cytometry. Gating strategy was according to Shawn Rose et al (40), in short, gated on 7-aad-/CD19-/CD3- and followed by CD11c+ for CD11c+ group, CD11b+ for CD11b+ group, F4-80 +/-/ Ly6G+ for neutrophils, F4-80+/ Ly6G-/Ly6C+/-, SSC high for eosinophils, F4-80+/ Ly6G-/Ly6C+, SSC low monocyte/macrophage type I, F4-80+ /Ly6G-/Ly6C-, SSC low for monocyte/macrophage type II. Four mice per group. Data shown are from one out of two experiments with similar results. Statistical analysis performed with Sidak’s multiple comparisons test, showed that all groups were not significantly different from each other.