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. 2018 Feb 28;118(7):3559–3607. doi: 10.1021/acs.chemrev.7b00570

Figure 8.

Figure 8

Thermostability of the mitochondrial ADP/ATP carrier and uncoupling protein in different detergents. Carrier unfolding was monitored by the fluorescence of CPM-adduct formation at cysteine residues as they become solvent-exposed due to thermal denaturation.153,154 (A) Thermal denaturation profile (top) and corresponding first derivative (bottom) of native yeast ADP/ATP carrier AAC3 diluted into assay buffer in DDM in the absence (solid line) or presence (dashed line) of CATR. (B) Same as in (A), but with AAC3 diluted in DPC. (C) Apparent melting temperatures (TM) of native yeast ADP/ATP carrier AAC2 with or without bound CATR diluted in octyl to tridecyl maltoside (8M-13M), Cymal4–7, dodecyl and decyl maltose neopentyl glycol (12MNG and 10MNG), octyl glucose neopentyl glycol (8GNG), LAPAO, and DPC. (D) Thermal denaturation profile of native uncoupling protein UCP1 in decyl-maltose neopentyl glycol (10MNG) (top) and corresponding first derivative (bottom) in the absence (solid line) or presence (dashed line) of GDP. (E) Same as in (D), but with native UCP1 in DPC. The apparent melting temperatures are reported in the derivative profiles. Data are from refs (146) and (154).