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. Author manuscript; available in PMC: 2018 Jul 6.
Published in final edited form as: Biochem J. 2017 Jul 6;474(14):2389–2403. doi: 10.1042/BCJ20160969

Figure 7. Cyclic nucleotide binding of PKA RIα CNB-A mutants.

Figure 7

Binding curves as derived from SPR solution competition experiments. (A) The PKA hRIα 115–274 double-mutant T192R/A212T has a switched selectivity compared with the wild-type CNB-A (black- and red-dashed lines for cAMP and cGMP, respectively). (B) The T192R mutant and (C) the A212T single mutants have a reduced affinity for cAMP and an increased affinity for cGMP compared with the wild-type CNB-A.