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. Author manuscript; available in PMC: 2018 Jul 6.

Published in final edited form as: Biochem J. 2017 Jul 6;474(14):2389–2403. doi: 10.1042/BCJ20160969

Table 1.

EC₅₀ values of PKA hRIα CNB domain constructs

His-PKA hRIα	EC₅₀ ± SD¹ (nM)		Ratio EC₅₀ (cAMP)/EC₅₀ (cGMP)

	cAMP	cGMP
CNB-A (115–274)
Wild type	151 ± 39	4554 ± 657	0.03
T192R	859 ± 197	1529 ± 53	0.56
A212T	420 ± 11	83 ± 33	5.06
T192R/A212T	5410 ± 1290	24 ± 5	225
CNB-B (234–381)
Wild type	3.6 ± 1.4	396 ± 25	0.009
V315L	7.5 ± 1.8	678 ± 100	0.011
G316R	15.3 ± 4.9	98 ± 41	0.156
A336T	4.9 ± 1.9	2.0 ± 0.1	2.45
V315L/G316R	11.5 ± 7.6	66 ± 2	0.174
G316R/A336T	15.8 ± 1.3	0.4 ± 0.1	39.5
V315L/G316R/A336T	10 ± 1	0.6 ± 0.3	16.7

¹

Mean values with standard deviation (SD) of at least four replicates from N ≥ 2 independent protein preparations.