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. 2018 Apr 10;4:2333721418768998. doi: 10.1177/2333721418768998

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Expression of SR Ca²⁺ handling proteins.

Note. (a) Upper part representative immunoblots obtained by antibodies specified in Figure 3b and lower part by Actin. Number of gels analyzed and replica are described in methods section. Pretreated values were set to one. Comparison between pre- and posttrained samples was performed by the nonparametric matched Wilcoxon test (W significative * for p < .05) except for Sarcalumenin which satisfied the criteria for paired Student’ T test (δ p < .05). (c) RNA analysis. Comparison between pre- and posttraining expression of indicated genes. Values are expressed as mean (n = 15) and vertical error bars represent SE; messenger RNA levels were normalized to GAPDH by the delta Ct method and then normalized to pre-ES samples which were set to one. SR = sarcoplasmic reticulum; ES = electrical stimulation.