Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| antibody | mouse monoclonal Alexa Fluor-488 conjugated antibody against NeuN |
Merck Millipore | MAB377X | 1:200 dilution |
| antibody | rabbit polyclonal primary antibody against GABA |
Sigma-Aldrich | A2052 | 1:1000 dilution |
| antibody | Alexa Fluor-488 goat anti-rabbit IgG secondary antibody |
Merck Millipore | A11008 | 1:500 dilution |
| chemical compound, drug | Isoflurane | Abbott | B506 | |
| chemical compound, drug | Urethane | Fluka analytical | 94300 | |
| chemical compound, drug | Fluorogold | Fluorochome, LLC | 52–9400 | |
| chemical compound, drug | Biotinylated dextran amine, 10.000 MW |
Thermo Fisher Scientific | D1956 | |
| commercial assay or kit | NucleoBond PC 100 | Macherey-Nagel | 740573 | |
| strain, strain background (mouse, both genders) |
C57Bl/6J | Universitätsklinikum Hamburg-Eppendorf – Animal facility |
C57Bl/6J | https://www.jax.org/strain/008199 |
| strain, strain background (mouse, both genders) |
Tg(dlx5a-cre)1Mekk/J | The Jackson Laboratory | Tg(dlx5a-cre)1Mekk/J | https://www.jax.org/strain/017455 |
| strain, strain background (mouse, both genders) |
R26-CAG-LSL- 2XChETA-tdTomato |
The Jackson Laboratory | R26-CAG-LSL-2XChETA- tdTomato |
https://www.jax.org/strain/021188 |
| strain, strain background (mouse, both genders) |
Ai40(RCL-ArchT/EGFP)-D | The Jackson Laboratory | Ai40(RCL-ArchT/EGFP)-D | |
| recombinant DNA reagent | pAAV-CAG-ChR2 (E123T/T159C) −2AtDimer2 |
Provided by T. G. Oertner | pAAV-CAG-ChR2 (E123T/T159C) −2AtDimer2 |
http://www.oertner.com/ |
| recombinant DNA reagent | pAAV-CAG-tDimer2 | Provided by T. G. Oertner | pAAV-CAG-tDimer2 | http://www.oertner.com/ |
| software, algorithm | Matlab R2015a | MathWorks | Matlab R2015a | https://www.mathworks.com |
| software, algorithm | Offline Sorter | Plexon | Offline Sorter | http://www.plexon.com/ |
| software, algorithm | ImageJ 1.48 c | ImageJ | ImageJ 1.48 c | https://imagej.nih.gov/ij/ |
| software, algorithm | SPSS Statistics 21 | IBM | SPSS Statistics 21 | https://www.ibm.com/analytics/us/en/technology/spss/ |
| software, algorithm | Cheetah 6 | Neuralynx | Cheetah 6 | http://neuralynx.com/ |
| other | Arduino Uno SMD | Arduino | A000073 | A000073 |
| other | Digital Lynx 4SX | Neuralynx | Digital Lynx 4SX | http://neuralynx.com/ |
| other | Diode laser (473 nm) | Omicron | LuxX 473–100 | |
| other | Electroporation device | BEX | CUY21EX | |
| other | Electroporation tweezer-type paddles |
Protech | CUY650-P5 | |
| other | Recording electrode (1 shank, 16 channels) |
Neuronexus | A1 × 16–3 mm-703-A16 | |
| other | Recording optrode (1 shank, 16 channels) |
Neuronexus | A1 × 16–5 mm-703-OA16LP | |
| other | Digital midgard precision current source |
Stoelting | 51595 |