Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
gene (Gaussia princeps)	gLUC	PMID: 18408930	AY015993.1	wild-type
gene (Gaussia princeps)	gLUC	PMID: 18408930	EU372000	human codon-optimised
cell line (Trypanosoma brucei)	2T1	PMID: 16182389
transfected construct (Trypanosoma brucei)	pRPa-iSL plasmid	PMID: 18588918	69244	available from addgene.org
transfected construct (Trypanosoma brucei)	pRPa plasmid	PMID: 18588918
transfected construct (Trypanosoma brucei)	pRPa-λ plasmid	this paper		see materials and methods
antibody	α-gLUC	New England Biolabs		one in 1000
sequence-based reagent	EUluc5 oligonucleotide	this paper		GATCCTGCAGCTCGAGATGAAGCCCACCGAGAACAACG
sequence-based reagent	EUluc3 oligonucleotide	this paper		GATCGAATTCAGATCTAAGCTTTTACAGCTTCGAGTCGCCGCCGGCGCC
sequence-based reagent	WTluc5 oligonucleotide	this paper		GATCCTCGAGATGAAACCAACTGAAAACAATG
sequence-based reagent	WTluc3 oligonucleotide	this paper		GATCAAGCTTTTATAATTTACTATCACCACCGGCACCCTT
sequence-based reagent	Lambda5 oligonucleotide	this paper		GATCAAGCTTTGCAGGGTGAGATTGTGGC
sequence-based reagent	Lambda3 oligonucleotide	this paper		GATCGAATTCGCTCAGTTGTTCAGGAATATG
sequence-based reagent	TUBF oligonucleotide	this paper		AGATCTTCAAACACTAGTTTAAGC
sequence-based reagent	TUBR oligonucleotide	this paper		CATGATAAATAAATAGAAGTGCTTTGTTG
sequence-based reagent	λF oligonucleotide	this paper		GATTCATAAGTTCCGCTGTGTGCCGCATCTC
sequence-based reagent	λR oligonucleotide	this paper		GCTCAGTTGTTCAGGAATATGGTGCAGCAG
commercial assay or kit	BioLux Gaussia luciferase	New England Biolabs
software, algorithm	Bowtie 2	PMID: 22388286
software, algorithm	SAMtools	PMID: 19505943
software, algorithm	edgeR	PMID: 19910308
software, algorithm	CAI calculator	http://www.umbc.edu//codon/cai/cais.php
software, algorithm	ANACONDA	http://bioinformatics.ua.pt/software/anaconda/
online database	TriTrypDB, RRID:SCR_007043	http://tritrypdb.org/tritrypdb/