TABLE 1:
Localization of GFP-Cps1.
| GFP-Cps1 localization (% of cells)a | ||
|---|---|---|
| Strain | Vacuole lumen | Vacuole membrane |
| WT | 97.0 ± 0.8 | 3.0 ± 0.8 |
| cdc48-10 | 11.7 ± 2.5 | 88.3 ± 2.5 |
| cdc48-10+DDI1b | 84.0 ± 3.7 | 16.0 ± 3.7 |
| cdc48-10+RAD23c | 70.3 ± 3.3 | 29.7 ± 3.3 |
| npl4-2 | 24.7 ± 3.9 | 75.3 ± 3.9 |
| npl4-2+DDI1b | 73.7 ± 4.5 | 26.3 ± 4.5 |
| npl4-2+RAD23c | 75.7 ± 1.2 | 24.3 ± 1.2 |
| ufd1-1 | 26.0 ± 4.5 | 74.0 ± 4.5 |
| ufd1-1+DDI1b | 63.3 ± 3.7 | 36.7 ± 3.7 |
| ufd1-1+RAD23c | 69.0 ± 7.9 | 31.0 ± 7.9 |
| ddi1Δ | 31.3 ± 6.6 | 68.7 ± 6.6 |
| ddi1Δ+DDI1b | 98.3 ± 0.9 | 1.7 ± 0.9 |
| ddi1Δ+RAD23c | 69.3 ± 3.7 | 30.7 ± 3.7 |
| dsk2Δ | 35.7 ± 2.9 | 64.3 ± 2.9 |
| rad23Δ | 92.0 ± 2.1 | 8.0 ± 2.1 |
| ddi1Δ dsk2Δ rad23Δ | 14.3 ± 1.7 | 85.7 ± 1.7 |
aLocalization is based on scoring 100 cells; n = 3 experiments. Average ± SD is given.
bComplemented with DDI1 expressed from a 2μm plasmid.
cComplemented with RAD23 expressed from a 2μm plasmid.