Table 1. Rates of transition between all observed FRET states for all three HIV-1 Envs.
The distribution of dwell times in each FRET state, determined through Hidden Markov Modeling (HMM), were fit to the sum of two exponential distributions (y = A1 exp –k1t + A2 exp –k2t) (Figure 4—figure supplements 1–3). The weighted average of the two rate constants from each fit are presented. Error bars represent 95% confidence intervals propagated from the kinetics analysis.
| HIV-1NL4-3 | k1→2 (s−1) | k2→1 (s−1) | k2→3 (s−1) | k3→2 (s−1) |
|---|---|---|---|---|
| Unliganded | 1.50 ± 0.04 | 3.05 ± 0.04 | 1.89 ± 0.05 | 1.32 ± 0.04 |
| sCD4 | 2.10 ± 0.04 | 1.52 ± 0.04 | 1.21 ± 0.03 | 1.85 ± 0.03 |
| sCD4D1D2-Igαtp | 1.60 ± 0.02 | 2.84 ± 0.04 | 1.87 ± 0.04 | 1.13 ± 0.02 |
| HIV-1JR-FL | ||||
| Unliganded | 1.30 ± 0.03 | 2.21 ± 0.03 | 1.70 ± 0.02 | 1.25 ± 0.02 |
| sCD4 | 1.52 ± 0.03 | 1.76 ± 0.04 | 2.25 ± 0.03 | 1.37 ± 0.05 |
| sCD4D1D2-Igαtp | 2.02 ± 0.06 | 2.06 ± 0.05 | 1.81 ± 0.06 | 1.06 ± 0.03 |
| HIV-1BG505 | ||||
| Unliganded | 1.10 ± 0.04 | 4.37 ± 0.08 | 2.86 ± 0.05 | 1.77 ± 0.03 |
| sCD4 | 1.21 ± 0.03 | 4.32 ± 0.08 | 1.81 ± 0.09 | 2.79 ± 0.04 |
| sCD4D1D2-Igαtp | 1.62 ± 0.06 | 2.82 ± 0.05 | 2.92 ± 0.04 | 2.31 ± 0.05 |