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. 2018 Mar 21;7:e33285. doi: 10.7554/eLife.33285

Figure 7. S1PR3 is dispensable for development of chronic mechanical hypersensitivity.

(A) Thermal latency before and after CFA treatment (indicated by dotted line); pgenotype = 0.0053 (two-way ANOVA; N = 5 mice per genotype). Sidak’s multiple comparison between genotypes for specific time points indicated on graph. Error bars represent mean ± SD. (B) (Left) Normalized 50% withdrawal threshold before and after CFA treatment (indicated by dotted line); p(genotype)<0.001 (two-way ANOVA). (Right) 50% withdrawal thresholds for same experiment (p(genotype)=0.1634; two-way ANOVA). (C) (Left) Thermal latency assessed before (‘Baseline’) and 24 hr post CFA injection with either vehicle (CFA + VEH) or TY 52156 (CFA + TY) acutely administered; p<0.0001 (one-way ANOVA N = 5 mice per treatment). (Right) Thermal latency assessed before and after CFA injection with either vehicle (CFA + VEH) or SKI II (CFA + SKI II) acutely administered on Day 1; p<0.0001 (one-way ANOVA; N = 5–7 mice per treatment). Dunnett’s test comparisons to baseline are indicated on graph. Error bars represent mean ± SD. (D) (Left) 50% withdrawal threshold assessed before and 24 hr post CFA injection with either vehicle (CFA + VEH) or TY 52156 (CFA + TY) acutely administered on Day 1; p<0.0001 (one-way ANOVA; N = 5 mice per treatment). Dunnett’s test comparisons to baseline are indicated on graph. (Right) 50% withdrawal threshold assessed before and 24 hr post CFA injection with either vehicle (CFA + VEH) or SKI II (CFA + SKI II) acutely administered; p-values indicated on graph (two-tailed unpaired t-test; N = 5 mice per group.

Figure 7—source data 1. S1PR3 is dispensable for development of chronic mechanical hypersensitivity.
Related to Figure 7.
elife-33285-fig7-data1.xlsx (44.3KB, xlsx)
DOI: 10.7554/eLife.33285.018

Figure 7.

Figure 7—figure supplement 1. S1PR3 KO animals display normal CFA-evoked immune cell recruitment.

Figure 7—figure supplement 1.

Related to Figure 7. (A) Recruitment of neutrophils (Neut.) or inflammatory monocytes (IMs) to hindpaw skin 24 hr post-CFA administration in S1PR3 HET and KO mice, as a dot plot of CD11b + cells plotting Ly6G fluorescence intensity vs. Ly6C intensity (AFU). Boxes are for illustrative purposes. (B) Recruitment of immune cells, including neutrophils (Neut.) and inflammatory monocytes (IMs) to hindpaw skin 24 hr post-CFA administration in HET and KO mice, displayed as total number of cells; N = 8 mice per genotype. Sidak’s multiple comparisons were made between HET and KO for each cell type.