Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| strain, strain background (C57BL/6J) |
C57BL/6J; WT; wild-type | The Jackson Laboratory | Jackson Stock #: 000664; RRID:IMSR_JAX:000664 |
|
| strain, strain background (B6.129S6- S1pr3tm1Rlp/Mmnc) |
S1PR3 KO; S1pr3-/- | MMRRC Repository; https://www.ncbi.nlm.nih.gov/pubmed/15138255; PMID: 15138255 |
B6.129S6-S1pr3tm1Rlp/ Mmnc; MMRRC Stock #: 012038-UNC; RRID: MMRRC_012038-UNC |
|
| strain, strain background (B6.Cg- S1pr3tm1.1Hrose/J) |
S1pr3-mCherry;
S1pr3mcherry/+ |
The Jackson Laboratory | B6.Cg-S1pr3tm1.1Hrose/J; Jackson Stock #: 028624; RRID:IMSR_JAX:028624 |
|
| antibody (Living Colors DsRed Rabbit Polyclonal Antibody) |
Rabbit anti-DsRed | Clontech | RRID:AB_10013483; Cat # 632496 |
|
| antibody (Chicken polyclonal to Neurofilament heavy polypeptide) |
Chicken anti-NefH | Abcam | RRID:AB_304560; Cat # ab4680 |
|
| antibody (Chicken polyclonal to beta III Tubulin) |
Chicken anti-β-tubulin III |
Abcam | RRID:AB_10899689; Cat # ab107216 |
|
| antibody (Mouse monoclonal [13C4/I3 C4] to PGP9.5) |
Mouse anti-PGP9.5 | Abcam | RRID:AB_306343; Cat # ab8189 |
|
| antibody (Rabbit polyclonal to EDG3) |
Rabbit anti-S1PR3 | Abcam | RRID:AB_732070; Cat # ab38324 |
|
| antibody (Mouse monoclonal to NF200) |
Mouse anti-NF200 | Sigma-Aldrich | RRID:AB_260781; Cat # N5389 |
|
| antibody (Chicken polyclonal to Peripherin) |
Chicken anti-Peripherin | Abcam | RRID:AB_777207; Cat # ab39374 |
|
| antibody (Goat Anti-Mouse IgG H and L Alexa Fluor 488) |
Goat anti-Mouse Alexa 488 |
Abcam | RRID:AB_2688012; Cat # ab150117 |
|
| antibody (Goat anti-Chicken IgY (H + L) Secondary Antibody, Alexa Fluor 488) |
Goat anti-Chicken Alexa 488 |
ThermoFisher Scientific | RRID:AB_2534096; Cat # A-11039 |
|
| antibody (Goat anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 594) |
Goat anti-Rabbit Alexa 594 |
Invitrogen | RRID:AB_2556545; Cat # R37117 |
|
| sequence-based reagent | S1pr3 Type I Probe | ThermoFisher Scientific; Affymetrix |
Assay ID: VB1-19668-VC | |
| sequence-based reagent | Scn1a Type 6 Probe | ThermoFisher Scientific; Affymetrix |
Assay ID: VB6-18173-VC | |
| sequence-based reagent | Npy2r Type 6 Probe | ThermoFisher Scientific; Affymetrix |
Assay ID: VB6-3197254-VC |
|
| sequence-based reagent | Piezo2 Type 6 Probe | ThermoFisher Scientific; Affymetrix |
Assay ID: VB6-18046-VC | |
| sequence-based reagent | Trpv1 Type 6 Probe | ThermoFisher Scientific; Affymetrix |
Assay ID: VB6-18246-VC | |
| sequence-based reagent | Trpa1 Type 6 Probe | ThermoFisher Scientific; Affymetrix |
Assay ID: VB6-16610-VC | |
| commercial assay or kit (ViewRNA ISH Tissue Assay Kit (2-plex)) |
ViewRNA ISH Tissue Assay Kit |
ThermoFisher Scientific; Affymetrix |
Cat # QVT0012 | |
| chemical compound, drug (Sphingosine-1-phosphate) |
Sphingosine 1-phosphate; S1P |
Tocris Bioscience; Avanti Polar Lipids |
CAS 26993-30-6; Cat # 1370; Cat # 860641 |
|
| chemical compound, drug (TY 521256) |
TY 52156 | Tocris Bioscience | CAS 934369-14-9; Cat # 5328 |
|
| chemical compound, drug (SKI II) |
SKI II | Tocris Bioscience | CAS 312636-16-1; Cat # 2097 |
|
| chemical compound, drug (Histamine dihydrochloride) |
Histamine | Sigma-Aldrich | CAS 56-92-8; Cat # H7250 |
|
| chemical compound, drug (Chloroquine diphosphate) |
Chloroquine | Sigma-Aldrich | CAS 50-63-5; Cat # C6628 |
|
| chemical compound, drug (E-Capsaicin) |
Capsaicin | Tocris Bioscience | CAS 404-86-4; Cat # 0462 |
|
| chemical compound, drug (Dimethyl sulfoxide) |
DMSO | Sigma-Aldrich | Cat # 8418–100 mL | |
| chemical compound, drug (Methanol) |
Methanol | Sigma-Aldrich | CAS 67-56-1; Cat # 34860 |
|
| chemical compound, drug (Linopirdine dihydrochloride) |
Linopirdine | Tocris Bioscience | CAS 113168-57-3; Cat # 1999 |
|
| chemical compound, drug (XE 991 dihydrochloride) |
XE 991 | Tocris Bioscience | CAS 122955-13-9; Cat # 2000 |
|
| chemical compound, drug (W146) |
W146 | Tocris Bioscience | CAS 909725-61-7; Cat # 3602 |
|
| chemical compound, drug (Freund's Adjuvant, Complete) |
Complete Freund's Adjuvant; CFA |
Sigma-Aldrich | Cat # F5881 | |
| chemical compound, drug (Formaldehyde, 16%, methanol free, Ultra Pure) |
Paraformaldehyde; PFA |
Polysciences, Inc. | Cat # 18814–10 | |
| chemical compound, drug (Tissue Tek Optimal cutting temperature compound (OCT)) |
OCT | Sakura Finetek USA | Cat # 4583 | |
| chemical compound, drug (Triton X-100 solution) |
Triton X-100 | BioUltra | CAS 9002-93-1; Cat # 93443 |
|
| chemical compound, drug (Phosphate-buffered saline (PBS), pH 7.4) |
PBS | Gibco | Cat # 10010023 | |
| chemical compound, drug (Benzyl benzoate) |
Benzyl benzoate | Sigma-Aldrich | CAS 120-51-4; Cat # B6630 |
|
| chemical compound, drug (Benzyl alcohol) |
Benzyl alcohol | Sigma-Aldrich | CAS 100-51-6; Cat # 305197 |
|
| chemical compound, drug (Sucrose) |
Sucrose | Sigma-Aldrich | CAS 57-50-1; Cat # S0389 | |
| chemical compound, drug (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation) |
Aqua | ThermoFisher Scientific | Cat # L34957 | |
| chemical compound, drug (Isoflurane, USP) |
Isoflurane | Piramal | CAS 26675-46-7 | |
| chemical compound, drug (4',6-Diamidino-2- Phenylindole, Dihydrochloride) |
DAPI | ThermoFisher Scientific | CAS 28718-90-3; Cat # 1306 |
|
| chemical compound, drug (Fluoromount-G, with DAPI) |
Fluoromount-G, with DAPI |
ThermoFisher Scientific | Cat # 00-4959-52 | |
| antibody (CD117 (c-Kit) Monoclonal Antibody (2B8), Biotin) |
c-Kit-Biotin | eBioscience | RRID:AB_466569; Cat # 13-1171-82 |
|
| antibody (FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), PE, eBioscience) |
FceRI-PE | eBioscience | RRID:AB_10804885; Cat # 12-5899-42 |
|
| antibody (CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), PE-Cyanine7, eBioscience) |
CD49b-PECy7 | eBioscience | RRID:AB_469667; Cat # 25-5971-82 |
|
| antibody (Anti-Siglec-F-APC, mouse (clone: REA798)) |
SiglecF-APC | Miltenyi Biotech | RRID:AB_2653441; Cat # 130-112-333 |
|
| antibody (Streptavidin FITC) | SA-FITC | eBioscience | RRID:AB_11431787; Cat # 11-4317-87 |
|
| antibody (Ly-6C Monoclonal Antibody (HK1.4), PerCP- Cyanine5.5, eBioscience) |
Ly6C-PerCP | eBioscience | RRID:AB_1518762; Cat # 45-5932-82 |
|
| antibody (Pacific Blue anti- mouse/human CD11b Antibody) |
CD11b-PB | BioLegend | RRID:AB_755985; Cat # 101223 |
|
| antibody (Brilliant Violet 785 anti-mouse Ly-6G Antibody) |
Ly6G-BV785 | BioLegend | RRID:AB_2566317; Cat # 127645 |
|
| antibody (CD45.2 Monoclonal Antibody (104), Alexa Fluor 700, eBioscience) |
CD45.2-AF700 | eBioscience | RRID:AB_657752; Cat # 56-0454-82 |
|
| software, algorithm (Igor Pro version 6.3) |
IgorPro | WaveMetrics | https://www.wavemetrics.com/order/order_igordownloads6.htm | |
| software, algorithm (Microsoft Excel 2011) |
Microsoft Excel | Microsoft | https://www.microsoft.com/en-us/store/d/excel-2016-for-mac/ | |
| software, algorithm (pClamp 10) | pClamp | Axon | http://mdc.custhelp.com/app/answers/detail/a_id/18779/~/axon%E2%84%A2-pclamp%E2%84%A2-10-electrophysiology-data-acquisition-%26-analysis-software | |
| software, algorithm (MetaFluor 7.8) |
MetaFluor | Molecular Devices | https://www.moleculardevices.com/systems/metamorph-research-imaging/metafluor-fluorescence-ratio-imaging-software | |
| software, algorithm (MATLAB) | MATLAB | MathWorks | https://www.mathworks.com/downloads/ | |
| software, algorithm (FIJI) | FIJI | NIH | https://imagej.net/Fiji/Downloads | |
| software, algorithm (LabChart Software) |
LabChart Software | AD Instruments | https://www.adinstruments.com/products/labchart | |
| software, algorithm (Graphpad Prism 7) |
Graphpad Prism | Graphpad | https://www.graphpad.com/scientific-software/prism/ | |
| software, algorithm (FlowJo 10.4.2) |
FlowJo | FlowJo | https://www.flowjo.com/solutions/flowjo/downloads | |
| software, algorithm (custom) | custom-made software in MATLAB |
this paper | NA |
https://github.com/buh2003/SpikeSortingPCA_DBSCAN (Hoffman, 2018; copy archived at https://github.com/elifesciences-publications/SpikeSortingPCA_DBSCAN) |
| other (Bovine serum albumin, cold ethanol fraction, pH 5.2,≥96%) |
BSA | Sigma-Aldrich | CAS 9048-46-8; Cat # A4503 |
|
| other (Isolectin B4 (Bandeireia simplicifolia), FITC-conjugate) |
IB4-FITC; IB4 | Enzo Life Sciences | Cat # ALX-650–001F- MC05 |
|
| other (Normal Goat Serum) | NGS | Abcam | Cat # ab7481 | |
| peptide, recombinant protein (δ-theraphototoxin- Hm1a) |
Hm1a | other; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919188/; PMID: 4919188 |
NA | obtained from the laboratory of David Julius (UCSF) |