Figure 4. SERT and NET are not endocytosed and oligomerized in AIM-100 treated cells.

(A) PAE cells transiently or stably expressing GFP-SERT or GFP-NET were incubated with vehicle or 20 µM AIM-100 for 2 hr at 37°C. 3D images were acquired from living cells. Maximal intensity projections of z-planes of representative YFP images are shown. Scale bars, 10 µm. (B) PAE/cells stably expressing YFP-DAT, GFP-SERT or GFP-NET were incubated as in (A), and lysates were electrophoresed and probed by western blotting with the GFP antibody. M, monomers; O, oligomers.