Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Dopamine trasporter (Homo sapiens) |
human DAT | n/a | Q01959 | |
| Dopamine transporter (Mus musculus) |
mouse DAT | n/a | Q61327 | |
| Genetic reagent | Mouse transgenic knock-in HA-DAT |
Rao et al., 2012 (available from Jackson lab) |
B6.Cg-Sklca3tm1.1Asor/J; Stock #029381 |
|
| Recombinant DNA reagent | YFP-HA-DAT | Sorkina et al., 2006 | Addgene #90244 | |
| Recombinant DNA reagent | W63A/YFP-HA-DAT | Sorkina et al., 2009 | Addgene #90946 | |
| Recombinant DNA reagent | YFP-DAT | Sorkina et al., 2003 | Addgene #90228 | |
| Antibody | HA11 (mouse monoclonal) | BioLegend | clone 16B12 (MMS101P) RRID:AB_291261 |
|
| Antibody | DAT (rat monoclonal) | EMD Millipore | MAB369; RRID:AB_2190413 |
|
| Antibody | ubiquitin (rabbit polyclonal) | Sigma-Aldrich | U5379; RRID:AB_477667 |
|
| Antibody | EEA1 (mouse monoclonal) | BD Biosciences | BDB610457; RRID:AB_397830 |
|
| Antibody | CHC (rabbit polyclonal) | Abcam | ab21679; RRID:AB_2083165 |
|
| Antibody | GFP (rabbit polyclonal) | Abcam | ab290; RRID:AB_303395 |
|
| Antibody | Ack1 (mouse monoclonal) | Santa Cruz | A-11; sc-28336; RRID:AB_626629 |
|
| Antibody | dynamin-2 (rabbit polyclonal) | ABR ((Thermo Fisher Scientific)) |
Cat # PA1-661; RRID:AB_2293040 |
|
| Antibody | GFP (mouse monoclonal) | Clontech | JL8; Cat# 632380; RRID:AB_10013427 |
|
| Antibody | Cy5 (donkey-anti-mouse) | Jackson Immuno Research | 715-175-151; RRID:AB_2340820) |
1:50 in antibody uptake assay |
| Antibody | Cy3 (donkey anti-mouse) | Jackson Immuno Research | 715-165-151; RRID:AB_2315777 |
1:500 in antibody uptake assay |
| Antibody | Alexa488 (donkey anti-rat) | Jackson Immuno Research | 712-545-153; RRID:AB_2340684 |
1:500 |
| Antibody | IRDye-800 (goat anti mouse) | LI-COR | 926–32210; RRID:AB_621842 |
1:20000 |
| Antibody | IRDye-680 (goat anti mouse) | LI-COR | 926–32220; RRID:AB_621840 |
1:20000 |
| Chemical compound, drug | Tfn-TxR (transferrin-Texas Red) | Invitrogen | Cat #T2875 | |
| Chemical compound, drug | LT-Red (LysoTrackerRed) | Invitrogen | Cat# L7528 | |
| Chemical compound, drug | AIM-100 | EMD MilliporeSigma | Cat#104833 | |
| Chemical compound, drug | KRCA-0008 | EMD MilliporeSigma | Cat# SML1304 | |
| Chemical compound, drug | PMA | EMD MilliporeSigma | Cat# P8139 | |
| Chemical compound, drug | Go6976 | EMD MilliporeSigma | Cat# 365250 | |
| Chemical compound, drug | Latranculin B | EMD MilliporeSigma | 428020 | |
| Transfected construct | siRNA to Ack1 | Integrated DNA Technologies Inc. |
Cat# 87793753; 87793756 |
|
| Transfected construct | siRNA to CHC | Thermo Fisher Scientific | Sorkina et al., 2005 | |
| Cell line (human) | SH-SY5Y | ATCC | RRID:CVCL_0019 | |
| Cell line (human) | HEK293T | ATCC | RRID:CVCL_0063 | |
| Cell line (human) | HeLa | ATCC | RRID:CVCL_0030 | |
| Cell line (porcine) | PAE | Westermark et al., 1990 | PMID: 2153283 | |
| Software | SlideBook6 | Intelligent Imaging Innovations, Inc. |
||
| Software | Odyssey Application Software 3.0 | Li-COR, Inc. |