Table 4. Ploidy level estimation of the reference M. anguillicaudatus using the five diagnostic ESR-SSR markersa.
| Locus | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MATS2-33 | Observed-allele No. | 2 | 2 | 2 | 2 | 2 | 1 | 2 | 3 | 3 | 2 | 4 | 2 | 3 | 3 | 2 |
| Ratio | 1.02/1 | 1.03/1 | 1.06/1 | 1.04/1 | 1.03/1 | — | 2.01/1 | 1.11/1.08/1 | 1.08/1.09/1 | 1/1.93 | 1.09/1.01/ 1/1.01 |
1/2.93 | 1.04/1.99/1 | 2.07/1/1.07 | 3.01/1 | |
| Calculated-allele No. | 2 | 2 | 2 | 2 | 2 | — | 3 | 3 | 3 | 3 | 4 | 4 | 4 | 4 | 4 | |
| MATS2-48 | Observed-allele No. | 1 | 1 | 1 | 2 | 2 | 1 | 3 | 2 | 2 | 3 | 4 | 3 | 2 | 1 | 3 |
| Ratio | — | — | — | 1.06/1 | 1.11/1 | — | 1.09/1/1.02 | 2.10/1 | 2.10/1 | 1.09/1.08/1 | 1.10/1.02/ 1.03/1 |
2.03/1.03/1 | 1/3.04 | — | 1.03/1.99/1 | |
| Calculated-allele No. | — | — | — | 2 | 2 | — | 3 | 3 | 3 | 3 | 4 | 4 | 4 | — | 4 | |
| MATS2-55 | Observed-allele No. | 1 | 2 | 2 | 2 | 2 | 3 | 3 | 3 | 1 | 3 | 2 | 3 | 4 | 3 | 3 |
| Ratio | — | 1.06/1 | 1.09/1 | 1.00/1 | 1.06/1 | 1.11/1.07/1 | 1/2.11 | 1/1.10/1 | — | 1.06/1.03/1 | 1.09/1 | 1/1.08/2.02 | 1.06/1.07/ 1.01/1 |
2.07/1/1.09 | 1.94/1/1.03 | |
| Calculated-allele No. | — | 2 | 2 | 2 | 2 | 3 | 3 | 3 | — | 3 | 2 | 4 | 4 | 4 | 4 | |
| MATS2-65 | Observed-allele No. | 1 | 2 | 2 | 1 | 2 | 2 | 2 | 3 | 1 | 2 | 3 | 2 | 1 | 3 | 4 |
| Ratio | — | 1.11/1 | 1.09/1 | — | 1.10/1 | 1.93/1 | 2.07/1 | 1.11/1.10/1 | — | 1.92/1 | 1.02/2.06/1 | 1.04/1 | — | 1/1.07/2.06 | 1.05/1.11/ 1.05/1 |
|
| Calculated-allele No. | — | 2 | 2 | — | 2 | 3 | 3 | 3 | — | 3 | 4 | 2 | — | 4 | 4 | |
| MATS2-74 | Observed-allele No. | 2 | 2 | 1 | 2 | 1 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 4 | 2 |
| Ratio | 1.07/1 | 1.12/1 | — | 1.07/1 | — | 1.09/1/1.06 | 1.09/1.03/1 | 1.09/1/1.05 | 1/1.11/1.09 | 1.12/1.06/1 | 1.07/2.07/1 | 1.91/1/1.03 | 1.10/1 | 1.09/1/1.10/1.00 | 1/1.10 | |
| Calculated-allele No. | 2 | 2 | — | 2 | — | 3 | 3 | 3 | 3 | 3 | 4 | 4 | 2 | 4 | 2 | |
| MS-Ploidy | 2n | 2n | 2n | 2n | 2n | 3n | 3n | 3n | 3n | 3n | 4n | 4n | 4n | 4n | 4n | |
| FCM-Ploidy | 2n | 2n | 2n | 2n | 2n | 3n | 3n | 3n | 3n | 3n | 4n | 4n | 4n | 4n | 4n |
a: Observed-allele No., the number of alleles directly recorded from electrophoretogram; Ratio, observed-allele quantitative values ratio, which was calculated by pairwise combinations between quantitative values for all allele-pairs at a particular locus; Calculated-allele No., the number of alleles obtained by combining the analysis of microsatellite banding patterns and the observed-allele quantitative values ratios; MS-Ploidy, ploidy level estimated by EST-SSRs; FCM-Ploidy, ploidy level determined by flow cytometry. In each locus, we considered the band with the lowest quantitative value representing a single dose allele, and were used as single-copy control for pairwise comparisons.