Figure 2.

Subcellular localization of Ni in leaves of hyper- and non-accumulator Thlaspi species as a percentage of total leaf Ni. Plants were exposed to 1 μm Ni (containing 40 μCi ⁶³Ni per μmol Ni) in a hydroponic solution for 1 d. Leaf samples were collected and fractionated into protoplasts and intact vacuoles. Ni localization was calculated as described in Figure 1. Values were averaged from three independent experiments ± sd. A, Subcellular distribution of Ni in T. goesingense. B, Subcellular distribution of Ni in T. arvense.