Table I.
Chlorophyll content and enzyme activities in leaf extracts and protoplast suspensions from plants exposed to 1 μm Ni for 1 d
| Plant | Chlorophyll
|
Enzymatic Activity
|
||||||
|---|---|---|---|---|---|---|---|---|
| Whole Leaf | Protoplast | Cytochrome c Oxidase | NADH-MDHa | |||||
| mg g−1 | μg per 106 protoplasts | μmol min−1 g−1 | μmol min−1 per 106 protoplasts | % of leaf total | μmol min−1 g−1 | μmol min−1 per 106 protoplasts | % of leaf total | |
| T. goesingense | 1.66 | 62.5 | n.d. | 0.016 | – | 144.2 | 6.7 | 123 |
| T. arvense | 1.21b | 49.8 | n.d. | 0.045 | – | 95.6 | 4.2 | 106 |
Values are means calculated from the results of three independent experiments. The proportions of enzymatic activities localized in the protoplasts were calculated by normalizing enzyme activities to the chlorophyll concentration in the respective fraction. n.d., Not determined.
a
MDH, Malate dehydrogenase.
b
Given value is the average from two experiments.