Table II.
Vacuolar yield and purity of vacuole-enriched fraction
| Plant | No. of Counted Vacuoles | Chlorophyll Content | NADH-MDHa | Cytochrome c Oxidase Activity |
|---|---|---|---|---|
| % of lysed protolasts | ||||
| T. goesingense | 22.2 ± 1.5 | 1.2 ± 0.1 | 2.0 ± 0.4 | 7.8 ± 4.3 |
| T. arvense | 22.1 ± 4.3 | 0.9 ± 0.5 | 2.2 ± 0.3 | 7.7 ± 6.0 |
No. of vacuoles, chlorophyll content, and marker enzyme activities were determined in the vacuole-enriched fraction, and these values are expressed as a percentage of the respective values measured in the total volume of protoplast suspension lysed to obtain the vacuoles. Values are means of all experiments ± se.
a
MDH, Malate dehydrogenase.