Fig 1. Overview of the TCR formats and triad of amino acids that form a stabilized interphase.

(A) Schematic presentation of the TCR formats. From left to right; scTCR wt, scTCR s, dsTCR, ct-dsTCR and cFab. TCR α- and β-chains are colored pale green and purple, respectively, and C_H1 and C_κ of the cFab are colored red and blue, respectively. The Vα/Vβ domains of the scTCRs are connected via a peptide linker as shown in green. Disulfide bonds are illustrated in yellow and the c-Myc and His6 -tags are shown in grey. (B) Top-down view of the amino acids forming the Vα/Vβ interphase triad, P50^TRAV, L50^TRBV and F103^TRBV. The crystal structure of the HLA-DQ2.5-DQ2.5-glia-α2-specific TCR S16 was used for modelling the triad (PDB ID 4OZH) [25].