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. 2018 Apr 2;14(4):e1007301. doi: 10.1371/journal.pgen.1007301

Fig 1. Presence, synthesis, and light-dependence of c-di-AMP in S. elongatus.

Fig 1

(A) Predicted CdaA protein topology. (B) Intracellular c-di-AMP measured by LC-MS for WT and cdaA transposon mutant (8S16-L9). Intracellular c-di-AMP concentrations were determined from raw quantities using cell volume (see Materials and Methods). The error bars represent standard error (SE) of five time points taken throughout a 24-h LDC in quadruplicate. ***P < 10−7 (Mann-Whitney-Wilcoxon Test). (C) C-di-AMP quantities in WT over one LDC. (D) C-di-AMP quantities upon the onset of darkness or light in WT. (C and D) C-di-AMP quantities are normalized by dividing by the average c-di-AMP concentration of the replicate. Error bars represent SE of four replicates.