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. 2018 Apr 2;14(4):e1007317. doi: 10.1371/journal.pgen.1007317

Fig 2. Yeast-two-hybrid experiments testing interactions between human FIGNL1, FLIP, RAD51 and DMC1 proteins.

Fig 2

Proteins of interest were fused with Gal4 DNA binding domain (BD, left) and with Gal4 activation domain (AD, top), respectively, and co-expressed in yeast cells. Full-length and truncated protein are schematically represented. For each combination, serial dilutions of yeast cells were spotted on non-selective medium (-LW), moderately selective media (-LWH) and more selective media (-LWHA). ++: Growth on both LWH and LWHA, interpreted as strong interaction. +: Growth on LWH and not on LWHA, interpreted as weak interaction. +*: Growth on LWH but cannot be interpreted as positive interaction because of auto-activation of one of the construct. Growth on neither LWH nor LWHA. n.d. Not determined. Pictures of yeasts are shown in S2 Fig.