Fig 9. FLIP genetically interacts with SDS.

A. Immunostaining of DMC1 (green) and the chromosome axis protein ASY1 (red) on leptotene/zygotene meiotic chromosome spreads. B. Quantification of DMC1 foci at leptotene/zygotene in sds, sds figl1 and sds flip. Each dot represents an individual cell and bars indicate the mean. C. Co-immunolocalization of ASY1 (red) and ZYP1 (green), which mark respectively chromosome axes and synapsed regions. Synapsis was partially restored in sds flip compared to single mutant sds. Scale bars 10μm. D. DAPI staining of chromosome spreads of male meiocytes at metaphase I and anaphase I. Scale bars 10μm. E. Fertility measured as number of seeds per fruit. Each dot represents a plant; at least 12 fruits per plant were analyzed. P values are the results of Fisher's LSD tests.