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. 2018 Apr 2;14(4):e1007317. doi: 10.1371/journal.pgen.1007317

Table 1. Tandem affinity purification using FIGL1 and FLIP as baits.

Two replicates of Tandem affinity purifications (TAP1 and TAP2) followed by mass spectrometry were performed using either FIGL1 (A) or FLIP (B) as a bait over-expressed in cultured cells. For filtering specific and false positive interactors, refer to Materials and Methods and [36]. The number of peptides and the fraction of the protein covered are indicated for each hit. Raw data are presented in S1 Table.

A Bait = FIGL1 TAP 1 TAP 2
protein name Number of peptides protein coverage % Number of peptides protein coverage %
FIGL1 47 70,5 42 62,3
FLIP 34 39,4 30 36,8
B Bait = FLIP TAP 1 TAP 2
protein name Number of peptides protein coverage % Number of peptides protein coverage %
FLIP 33 40,6 37 46,2
FIGL1 18 33,2 22 40,5