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. 2018 Apr 2;12(4):e0006351. doi: 10.1371/journal.pntd.0006351

Fig 2. Tc-STAMS2 approach tested against: 1) the CL14 T.cruzi strain, 2) T. vivax dataset and 3) LC-MS/MS datasets from E.coli and human and mouse placental tissues.

Fig 2

The sensitivity of Tc-STAMS2 approach was tested for the detection of intra-DTU strains such as CL14 and CLBrener strains belonging to DTU-VI. Moreover, the specificity of Tc-STAMS2 approach was tested for the assignment of MS/MS spectra derived from phylogenetically distant organisms such as mouse and human. In particular, the MS/MS spectral library was built using seven strains belonging to six DTUs such as Sylvio X10 cl1 (DTU-I), Y (DTU-II), M6241 cl6 (DTU-III), CanIII cl1 (DTU-IV), MN cl2 (DTU-V), CL Brener and CL14 (DTU-VI) strains and MS/MS data from T. vivax (epimastigote, metacyclic and bloodstream forms) were added to the spectral library [22]. Independent LC-MS/MS runs of the different T.cruzi strains, T.vivax life stages, human and mouse placenta tissue and E.coli were compared against the MS/MS spectral library using SpectraST software. Intra-DTU discrimination was achieved for CL14 and CL Brener and no assignment was made for the E.coli, mouse and human samples. MS/MS spectra from T.vivax were assigned specifically to T.vivax without identification of T.cruzi.