Figure 6. Precision of fixed particle tracking. ( a ) A fixed particle is tracked by 3D-DyPLoT at different count rates. The precision is ( b ) 17.6 nm in X, ( c ) 26.4 nm in Y, and ( d ) 53.4 nm in Z at an emission rate of 100 kHz. ( e ) When probing slower processes, the feedback control constant (KI) can be reduced by a factor of 10 to increase precision. Under this reduced feedback control, the precision is ( f ) 6.5 nm in X, ( g ) 8.3 nm in Y, and ( h ) 10.5 nm in Z at an emission rate of 100 kHz. Please click here to view a larger version of this figure.