(A) Quantification of the intensities of chromatin-bound RAD21-Myc in HeLa cells transfected with the indicated plasmids and siRNAs and synchronized in early S phase by thymidine. Each dot in the graph represents a single cell. Mean ± SD (Vector + siLuc, n = 73; Vector + siMCM2, n = 77; HA-MCM2 + siLuc, n = 52; HA-MCM2 +siMCM2, n = 48). (B) Quantification of the intensities of chromatin-bound RAD21-Myc in HeLa cells transfected with the indicated siRNAs and synchronized in early S phase by thymidine. Each dot in the graph represents a single cell. Mean ± SD (siLuc, n = 55; siMCM2, n = 78; siMCM3, n = 57; siMCM5, n = 51). (C) DAPI (blue), anti-HA (green), and anti-MCM5 (red) staining of HeLa cells transfected with vector or HA-MCM2. Scale bar, 5 μm. (D) Quantification of MCM5 intensities on chromatin of cells in C). Each dot in the graph represents a single cell. Mean ± SD (Vector, n = 14; HA-MCM2, n = 13). (E) Quantification of the STAG2 intensities on chromatin in HeLa cells transfected with the indicated siRNAs and synchronized in early S phase by thymidine. Each dot in the graph represents a single cell. Mean ± SD (siLuc, n = 89; siMCM2, n = 130; siNIPBL, n = 45). (F) Quantification of the STAG2 intensities on chromatin in telophase HeLa cells transfected with the indicated siRNAs. Each dot in the graph represents a single cell. Mean ± SD (siLuc, n = 74; siMCM2, n = 61; siNIPBL, n = 58).