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. Author manuscript; available in PMC: 2018 Jun 4.
Published in final edited form as: Prostate Cancer Prostatic Dis. 2017 Dec 4;21(1):113–125. doi: 10.1038/s41391-017-0013-x

Figure 2. Transcript-Level Evaluation of the Immune Components of the Post-Castration Tumor Microenvironment (TME).

Figure 2

Figure 2

Bulk tumor samples from animals treated as in Figure 1 analyzed by qPCR using indicated probe sets. Fold change calculated by ΔΔCt, normalized to Pre-C. Reactions performed in triplicate, repeated × 2.

(A) Transcripts associated with immune cell subsets

(B) Cytokines

(C) Immunologically relevant transcription factors

(D) Immune checkpoints and ligands

Data are mean ± SEM. P-values determined by unpaired t test, *, P <0.05; **, P <0.01; NS, not statistically significant.

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