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. Author manuscript; available in PMC: 2018 Jun 4.
Published in final edited form as: Prostate Cancer Prostatic Dis. 2017 Dec 4;21(1):113–125. doi: 10.1038/s41391-017-0013-x

Figure 3. Flow Cytometry (Protein-Level) Quantification of the Cellular Immune Components of the Post-Castration Tumor Microenvironment (TME).

Figure 3

Single cell suspensions of resected tumors before castration (Pre-C), day 7 post castration (Post-C D7), and upon development of castration resistance (C-Resistant) analyzed by flow cytometry. Data are representative of at least 3 independent experiments, with 5–8 animals per group.

(A) T cell populations as a percentage of CD45+ cells in the TME. Note that Treg are shown as a percentage of CD45+ / CD4+ cells for clarity.

(B) Additional populations of interest, note that here macrophages (MAC) are defined as CD45 cells that are CD11b+ F4/80+.

C) Summary data with Treg as a percentage of CD4 expanded for clarity.

*, P<0.05; **, P<0.01; **, P<0.001; and ****, P<0.0001; NS, not statistically significant.

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