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HPLC profiles of AO separation. a Preparative HPLC profile of the AO mixture. A total of 12 fractions collected in 110 min are shown. b Analytical HPLC profile of the AO mixture. Fraction F6, which was defined based on the preparative HPLC chromatogram, was divided into 2 fractions for analytical HPLC. c–n Analytical HPLC profiles of each oligosaccharide fraction collected by preparative HPLC. The purity of each fraction was calculated via the HPLC area normalization method
