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. 2018 Apr 12;8:5887. doi: 10.1038/s41598-018-24081-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Illustration of in vivo vascular lumens during angiogenesis. (A) Dextran perfusion of angiogenic in vivo microvascular networks identifies vascular lumens in capillary sprouts. (B,C) Higher magnifications of capillary sprouts in the tissue region, indicated by the above squares. Representative examples highlight the vascular lumen extending to the tip of a capillary sprout (B) as well as the vascular lumen forming behind the tip of the sprout (C). Arrows identify the distal edges of capillaries. Scale bar = 20 µm.