Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 12;8:5907. doi: 10.1038/s41598-018-24074-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Further characterization of RhiPSC-CMs. (a) Representative action potentials from patch clamp recordings of the three cardiomyocyte substypes (ventricular, n = 29; atrial, n = 16; and nodal, n = 7) at day 20 of differentiation. (b) Patch clamp recording of MDP, peak voltage, APD at different levels of repolarization (90% and 50%), and dV/dt_max rate from three cardiomyocyte subtypes (ventricular, n = 26; atrial, n = 19; and nodal, n = 7) at day 15–20 of differentiation. (c) Representative immunofluorescence staining for α-ACTININ from more than 3 experiments showed increased sarcomere alignment in RhiPSC-CMs from 10 days to 60 days of culture. The details within red rectangle boxes in upper panels are shown in the lower panels. Nuclei were stained by DAPI. Scale bars, 25 µm. (d) Co-staining for MLC2A and MLC2V with cTnI or cTnT showed that most cells expressed MLC2A at early stage (10 days) but expressed MLC2V at late stage (60 days), while a mix population of MLC2A and MLC2V expressing cells was observed at day 20. Nuclei were stained by DAPI. Scale bars, 25 µm.