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. 2018 Apr 12;9:1433. doi: 10.1038/s41467-018-03929-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — TCS tuning an E. coli aspartate sensor. a The Taz-OmpR aspartate sensing TCS in E. coli. The Taz SK is composed of the Tar sensing domain fused to the EnvZ DhP and CA domains. Taz signals to OmpR which controls transcription from the P_ompF output promoter. b The relationship between the phosphatase activity of the mutant as measured by Inouye and colleagues³⁶ and the K_1/2 fit parameter from transfer function fits in Supplementary Fig. 5. Error bars are the 95% confidence interval of the K_1/2 fit parameter