Table 1.
Cell proliferation assay
| Cell line | EBV status | P53 status | Roniciclib | Cisplatin | ||
|---|---|---|---|---|---|---|
| Mean IC 50 (nM) | SD | Mean IC 50 (μM) | SD | |||
| HONE-1 | − | Wild type | 38.41 | 4.19 | 2.75 | 0.33 |
| HK-1 | − | Mutant (exon 5, codon 130, CTC>GTC | 28.27 | 0.50 | 2.61 | 0.07 |
| C666-1 | + | Mutant (exon 7, codon 249 deletion) | 10.97 | 2.63 | 9.00 | 0.94 |
| CNE-2 | − | Mutant (exon 8, codon 280, AGA>ACA) | 31.02 | 3.09 | ||
| HCT116 +/+ | − | Wild type | 23.73 | 3.23 | ||
| HCT116 −/− | − | Wild type | 25.48 | 0.98 | ||
Cell lines were treated with roniciclib and cisplatin at various concentrations and the inhibitory concentrations were determined. Mean IC50 values were derived from three independent experiments; each concentration was done in triplicates. Respective mean IC50's and standard deviation (SD) were calculated
IC50 half-maximal inhibitory concentration, SD standard deviation