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. 2018 Apr 12;8:5898. doi: 10.1038/s41598-018-24273-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Concentrations of soluble complement markers after perfusion with NHS with or without complement inhibitors. Human soluble complement activation markers in the perfusate were detected by ELISA (human C5a) and Bio-Plex [human soluble (s)C5b-9]. Data are presented as scattered dot plot with mean values, error bars indicate standard deviations, NHS post-perfusion n = 5, NHS + C1INH n = 6, NHS + APT070 n = 7, NHS + DXS n = 5, p-values: **p < 0.01, ****p < 0.0001. Sera from different donors with different blood groups were used as pool of 8 donors.