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. 2018 Apr 12;8:5898. doi: 10.1038/s41598-018-24273-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of microchannel fabrication and pump connecting. PDMS is poured into a Petri dish (Ø 60 mm). Supporting and mold needles are placed as shown in the picture and the whole Petri dish is incubated overnight at 60 °C. Needles are removed, inlet and outlet holes are made with a 2 mm biopsy puncher. Lateral holes are sealed with more PDMS. The second and the third steps show the device inside a Ø 35 mm Petri dish for a demonstration purpose. Normally everything is done using a Ø 60 mm Petri dish which can host up to 4 microchips. After plasma oxygen treatment, the microchip is bounded to a glass slide, coated with fibronectin and collagen I, and ultimately cells are seeded within the microchannels. One day after seeding a peristaltic pump is connected and a shear stress of 10 dyn/cm² is applied.