Fig. 6.

Improved metabolic profile of HFD-fed mice after administration of chemically modified autophagy enhancer, MSL-7. a, b Eight-week-old male C57BL/6 mice were fed HFD or normal chow diet (NCD) for 8 weeks, and then treated with 50 mg/kg MSL-7 3 times a week for 8 weeks. Nonfasting blood glucose level (a) and body weight (b) were monitored (F = 56.5, df = 1 for a). c IPGTT was conducted after in vivo administration of MSL-7 for 8 weeks (F = 52.7, df = 1). d AUC of the IPGTT curve in c (F = 4.2, df treatment = 3, df residual = 33). e ITT was conducted after in vivo administration of MSL-7 for 8 weeks (F = 52.6, df = 1). f AUC of the ITT curve in e (F = 10.3, df treatment = 3, df residual = 33). g–m Metabolic parameters of mice treated with MSL-7. Fasting blood glucose level (g) and serum levels of insulin (h), C-peptide (j), leptin (k), NEFA (l), and adiponectin (m) were determined. HOMA-IR was calculated (i). All data in this figure are the means ± s.e.m. from ≥ 3 independent experiments performed in triplicate. *P < 0.05, **P < 0.01 and ***P < 0.001 by two-way ANOVA with Bonferroni’s post-hoc test (a, c, e), one-way ANOVA with Tukey’s post-hoc test (d, f) or two-tailed Student’s t-test (g–m)