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. 2018 Apr 12;8:5923. doi: 10.1038/s41598-018-23717-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical localisation of HuC/D (A,D) and neuronal NOS (B,E) in the myenteric plexus and circular muscle layer of distal colon from WT (A–C) and TLR2/4^−/− (D–F). The composite images (C,F) illustrate the proportion of HuC/D positive neurons that also express nNOS. Scale bar in F applies to all panels. Summary graphs compare number of HuC/D positive neurons (G), proportion of nNOS positive neurons (H) and ganglion area (I) in distal colon of WT and TLR2/4^−/−.