Figure 2.

(A) Illustration of post-translational import experiment. MTS, brown. (B) Aco1-N and endogenously co-expressed aconitase exhibit a defect in mitochondrial import. Yeast strains harboring plasmids encoding the indicated proteins were induced in galactose medium and pulse labelled with [³⁵S] methionine–cysteine and CCCP. An aliquot of these cultures was further incubated with DTT in order to restore post-translational import of proteins into mitochondria in the presence of cold methionine–cysteine. Total cell extracts were immunoprecipitated with Aco1, NFS1 and MDH1 antisera and SDS-PAGE followed by autoradiography was performed. Mature, m; Precursor, p.