Figure 7.

Model of the aconitase targeting mechanism in yeast. The aconitase N-terminal domains which are first translated on the ribosome, are initially poorly translocated into mitochondria. Upon translation of the whole protein, the C-terminal domain can then chaperone the N-terminal domains into a post translational mode of import. Ssa1/2 can affect targeting by binding aconitase and decreasing the interaction between the N and C terminal domains, thereby slowing import and causing more aconitase to end up in the cytosol. C- C terminal domain; N- N terminal domains; MTS- mitochondrial targeting signal.