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. 2018 Apr 12;8:5916. doi: 10.1038/s41598-018-24080-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MMP14 biosensor binding of MG2P dye on the EC surface is dynamic. (a) Whole-cell image of an EC expressing MMP14 biosensor in the presence of MG2P dye. White box represents the zoom regions shown in (b–e). Yellow circle is the region of photobleaching. (b–e) Zoomed regions from (a) showing example grayscale images of MMP14 biosensor, MG2P dye, and Overlay at defined times during the FRAP experiment: (b), pre-bleach; (c), immediately following the photo-bleach (t, 0); (d), half time of final measured fluorescence recovery (t, ½); (e), total time of final measured fluorescence recovery (t). (f) Fluorescence recovery graph for the MG2P dye (pink) and the MMP14-biosensor (blue). Vertical dashed lines represent the position of t,1/2 for MG2P dye (pink) and the MMP14-biosensor (blue). Scale bars = 20 µm. Error bars = + /− SD.