Figure 2.

Broad CD4 T cell specificity identified in the draining lymph node (dLN) is maintained at the site of infection. Female A/J mice were infected intranasally with 50,000 EID₅₀ of A/New Caledonia/20/99 H1N1 virus. The number of virus peptide-specific IL-2 producing (blue) and IFN-γ producing (green) CD4 T cells were quantified at days 10–11 postinfection in CD4 T cells using cytokine EliSpots. Cells isolated from mediastinal lymph nodes (mLN) are indicated in (A) and from the lung in (B). Results are represented as cytokine-producing spots per million CD4 T cells and represent mean ± SD of three independent experiments.