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. 2018 Apr 12;8:5917. doi: 10.1038/s41598-018-24027-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Elasticity of thin (left) and thick (right) wildtype myotubes. 3D-reconstruction of representative thin (A) and thick (B) myotubes, with elasticity coloration. Yellow color shows softer portions, while red to blue colors show stiffer regions. Color bar goes from 0 kPa (yellow) to 5 kPa (dark blue). (C and D) represent the elasticity distributions of force maps corresponding to A and B respectively. Light gray curves are single Gaussian functions, while black curves represent the sum of the two. Insets (E and F) show the optical images of the measured myotubes. (G) The bars represent the obtained average of the double Gaussians (Peak 1 and Peak 2), fitted on the elasticity value distributions of individual force maps, measured on thin and thick myotubes. In brackets the number of the analyzed force maps is denoted. Error bars are standard error of the mean. As indicated, significant difference was found on both peaks of the Gaussian fit (*p < 0.05, **p < 0.01, Mann-Whitney test).