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. 2018 Apr 12;8:5917. doi: 10.1038/s41598-018-24027-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Primary fusion of wildtype myotubes. Immunofluorescence images are presented with actin (green), myosin (red) and nucleus (blue) staining of wildtype myotubes at 7 DIV. (A) Primary fusion process can be observed while the actin rich projection of myoblast 1 (B, white arrows) is elongated along myotube 2 and seems to initiate fusion with it. Myoblasts 3–5 present fusion initiation with perpendicular orientation on the myotube by an actin rich contact region (C, white arrows). The enlargements (D and E) show the well separated striations of myotube 2 with myosin and actin staining, respectively.