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. 2017 Jan 2;130(2):227–238. doi: 10.1007/s10265-016-0899-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Sequence alignment of ADF. Saccharomyces cereviseae COF1 (ScCOF1), Homo sapiens COF1 (HsCOF1), Physcomitrella patens ADF (PpADF), Arabidopsis thaliana ADF1 and ADF7 (AtADF1 and AtADF7, respectively), and Oryza sativa ADF1 (OsADF1). Boxes indicate phosphorylation sites, and a black box indicates a nuclear localization sequence (NLS). Although angiosperm ADFs lack conventional NLS, their nuclear localization has been confirmed. Shaded residues indicate phosphatidylinositol 4,5-bisphosphate binding sites (Zhao et al. 2010), and residues indicated as bold characters are actin-binding sites (Dong et al. 2013)