Skip to main content
. 2018 Apr 6;9:343. doi: 10.3389/fphys.2018.00343

Figure 6.

Figure 6

Silencing ABCA1 significantly suppressed the inhibitory effect of TSF on PA-induced total cholesterol levels in mTECs. (A) Western blot analysis of ABCA1, PGC-1α, and LXR expression in mTECs cultured for 48 h, and then exposed to OA (oleic acid 50 μM), PA (sodium palmitate 50 μM), PA+T (PA 50 μM+ TSF 500 μg/mL), PA+T+A (PA 50 μM+ TSF 500 μg/mL+ Abca1-SiRNA), or PA+T+A (PA 50 μM+ TSF 500 μg/mL+ Co-Abca1-SiRNA). (B) Real-time PCR analysis of ABCA1, PGC-1α, and LXR expression in mTECs cultured for 6 h. (C) A colorimetric analysis of total cholesterol levels in the mTECs cultured with Abca1-SiRNA for 24 and 48 h. *P < 0.05, **P < 0.01 vs. the PA group; #P < 0.05, ##P < 0.01 vs. the PA+T+A group.