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. 2018 Apr 6;12:9. doi: 10.3389/fnsys.2018.00009

Figure 1.

Figure 1

CXC mRNA transcript expression in female mouse urothelium (A) and detrusor (B) and the effects of cyclophosphamide (CYP)-induced cystitis (4 h, 48 h, chronic) as determined by quantitative PCR (qRT-PCR). (A) CXCL9 and CXCL10 mRNA levels increased significantly (*p ≤ 0.01) in the urothelium with 4 h CYP treatment compared to control. CXCL10 mRNA expression also increased significantly (*p ≤ 0.01) in the urothelium with 48 h CYP treatment compared to control. CXCL9 and CXCL10 mRNA expression with acute 4 h CYP-induced cystitis was significantly (#p ≤ 0.01) greater than that observed with 48 h and chronic CYP treatment. No changes in CXCL11 mRNA expression were demonstrated in the urothelium with CYP treatment of any duration. (B) CXCL9 and CXCL10 mRNA levels were significantly (*p ≤ 0.01) increased in the detrusor of female mice with 4 h CYP treatment compared to control and these levels were significantly (#p ≤ 0.01) greater than those detected at the 48 h and chronic time points. CXCL10 mRNA expression in the detrusor of female mice was significantly (*p ≤ 0.01) increased with all CYP treatment durations compared to control. CXCL11 mRNA expression was significantly (*p ≤ 0.01) decreased in the detrusor of female mice with 48 h CYP treatment compared to control. CXC mRNA is expressed as the fold change of relative CXC expression/relative L32 expression and normalized to expression in urothelium (A) or detrusor (B) from control mice. Sample sizes are n = 6–7 for each group.