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. 2018 Apr 6;12:9. doi: 10.3389/fnsys.2018.00009

Figure 2.

Figure 2

CXC mRNA transcript expression in male mouse urothelium (A) and detrusor (B) and the effects of CYP-induced cystitis (4 h, 48 h, chronic) as determined by quantitative PCR (qRT-PCR). (A) CXCL9 and CXCL11 mRNA levels decreased significantly (*p ≤ 0.01) in the urothelium with 48 h and chronic CYP treatment compared to control and CXCL11 mRNA levels also significantly (*p ≤ 0.01) decreased in the urothelium with 4 h CYP treatment compared to control. CXCL10 mRNA expression also decreased significantly (*p ≤ 0.01) in the urothelium with 4 h and chronic CYP treatment compared to control. CXCL9 mRNA expression was significantly (#p ≤ 0.01) decreased with 48 h and chronic CYP treatment compared to 4 h CYP treatment. CXCL11 mRNA expression was progressively and significantly (*p ≤ 0.01) decreased with increasing duration of CYP treatment (4 h > 48 h > chronic CYP). (B) CXCL9 and CXCL11 mRNA levels were significantly (*p ≤ 0.01) decreased in the detrusor of male mice with 4 h CYP and chronic CYP treatment compared to control. CXCL10 mRNA expression in the detrusor of female mice was significantly (*p ≤ 0.01) increased with 4 h and 48 h CYP treatment durations compared to control (*) and chronic CYP treatment (#). CXCL11 mRNA expression was significantly (*p ≤ 0.01) increased in the detrusor of male mice with 48 h CYP treatment compared to control and this expression was significantly (#p ≤ 0.01) greater than CXCL11 mRNA expression at 4 h and chronic CYP treatment. CXC mRNA is expressed as the fold change of relative CXC expression/relative L32 expression and normalized to expression in urothelium (A) or detrusor (B) from control mice. Sample sizes are n = 6–7 for each group.