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. 2018 Apr 12;8:5863. doi: 10.1038/s41598-018-21807-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of H₂S on TGF-β1 signal pathway activated by 4.25% glucose PDFs in RPMCs. RPMCs were incubated with or without NaHS (50, 100, 300 μmol/L) for 30 min, followed by the stimulation of the mixture of 4.25% glucose PDFs and culture medium by 1:1 for 1 h. (a) Representative images of TGF-β1, phospho-Smad3, phospho-Smad2/3 are presented and (b) relative abundance of these proteins in each group are calculated. Data represent mean ± SD of three independent experiments. **P < 0.01 versus control group; ^#P < 0.05, ^##P < 0.01 versus 4.25% glucose PDFs group.