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. 2018 Apr 12;8:5927. doi: 10.1038/s41598-018-24257-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of SIRT7 in the kidney. (A) SIRT7 expression in mouse kidney, liver, and heart was evaluated. GAPDH and β-actin were used as loading controls. SIRT7 protein expression was deleted in Sirt7 KO mouse kidney. (B) SIRT7 expression in the kidney of Sirt7 FRT/floxed mutant mouse was evaluated by β-galactosidase staining. (C) Representative photomicrographs (×400) of immunohistochemical staining for SIRT7 in kidney sections. Scale bar: 50 µm. (D) Representative photomicrographs (×400) of double immunostaining (lectin and SIRT7). PHA-E was used as a marker of proximal tubules and DBA was used as a marker of collecting tubules. The arrows indicate SIRT7-expressing nuclei. Scale bars: 50 µm.