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. 2018 Apr 12;8:5880. doi: 10.1038/s41598-018-24043-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cluster analysis of microbial communities in the ileal mucosa of pigs fed control diet (CON), flaxseed meal (FM) and oat hulls (OH) diets. The sample identifiers on the top branches for diet treatments are coloured. Right branches are coloured to indicate the taxonomical assignment of the OTUs at the phylum level. Each row represents one bacterial taxa (relative abundance above 0.01%), and some taxa could only be classified to family (f), order (o), class (c), or phylum (p) level. The taxa names were labeled by the colour code (magenta) to indicate the abundance of the taxa significantly differed between diet treatments (P < 0.05). The normalized relative abundance of bacterial taxa from illumina-sequenced 16 S rRNA sequences in each sample is reflected by the colour of the scale (light yellow to black) on the heat map. The dendrogram on the top shows how the samples are clustered based on the Bray–Curtis dissimilarity measure, averaged by diet treatment. The dendrogram on the right shows clustering of bacterial taxa data based on the Spearman’s rank correlation. The Box-Plots showed the relative abundances of bacterial taxa in ileal mucosa microbiota among the three diet treatments.