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. 2018 Apr 6;9:667. doi: 10.3389/fmicb.2018.00667

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Copyright © 2018 Reis, Boff, Verly, Melo-Braga, Cortés, Santos, Pimenta, Amaral, Resende and de Lima.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification and mass spectrometry analyses of LyeTxI-b synthetic peptide. (A) The crudely synthesized peptide was first purified by RP-HPLC using Discovery^® Bio Wide Pore C18^TM column (Supelco) with a stepped gradient of acetonitrile in 0.1% TFA. (B) Purification of fraction B by RP-HPLC on PepMap C18 column (4.6 mm × 150 mm). The column was previously equilibrated with 0.1% aqueous TFA and the peptide eluted with a linear gradient of acetonitrile in 0.1% TFA. (C) LyeTxI-b was analyzed by MALDI-TOF-TOF mass spectrometry and (D) the primary sequence was confirmed by MS/MS using LIFT fragmentation.